曹云¹，曹文涛^1,2*，王晓丹^1,3，杨文娟¹，刘云茂²

（1. 贵州大学酿酒与食品工程学院，贵州贵阳 550025：

2.贵州轻工职业技术学院轻工化工系，贵州贵阳 550025；

3.贵州大学贵州省发酵工程与生物制药重点实验室，贵州贵阳 550025）

摘要：为了筛选耐高温产酒精酵母菌，以贵州省茅台镇酱香型酒厂的第四轮次酒醅为实验材料，采用平板划线分离法筛选出37株酵母菌，从37株酵母菌中通过热致死温度、TTC染色法获得1株耐高温且高产酒精的J18菌株，对其在最高发酵温度的耐乙醇、耐酸、耐糖及发酵力进行了研究。结果表明：J18菌株的最高发酵温度为44 ℃，耐乙醇浓度18%vol，最适生长pH值为5.5；最适生长葡萄糖浓度为35%，最适发酵条件下发酵力为4.881 g/100 mL。对该菌株进行形态学鉴定和26S rDNA测序，确定该菌株为酿酒酵母（Saccharomyces cerevisiae）。

关键词：耐高温；高产酒精；酿酒酵母

中图分类号：TS261.11     文献标识码：A      文章编号：1674-506X（2023）01-0083-0007

Screening,Identification and Characterization of Thermotolerant

Yeast Strains with High-yield of Alcohol

CAO Yun¹, CAO Wentao^1,2*, WANG Xiaodan^1,3, YANG Wenjuan¹, LIU Yummao²

(1.School of Liquor and Food Engineering, Guizhou University, Guiyang Guizhou 550025, China;

2.Department of Light Industry and Chemical Engineering, Guizhou Light Industry Technical College, Guiyang Guizhou 550025, China; 3.Key Laboratory of Fermentation Engineering and Biological Pharmacy of Guizhou Province, Guizhou University, Guiyang Guizhou 550025, China)

Abstract: In order to screen high temperature resistant alcohol-producing yeast, the fourth round of fermented grains of Maotai Town sauce-flavor liquor distillery in Guizhou province were used as experimental materials. 37 strains of yeast were screened by the plate streak separation method, and one strain J18 with high temperature tolerance and high yield of alcohol was obtained from the 37 strains by lethal superheat temperature and TTC staining method. The ethanol resistance, acid resistance, sugar resistance and fermentability of the strain at the highest fermentation temperature were studied. The results showed that the maximum fermentation temperature of strain J18 was 44 ℃, the tolerance to ethanol concentration was 18%vol, and the optimum pH value was 5.5. The optimal glucose concentration of growth was 35%; Under the optimum fermentation conditions, the fermentation power was 4.881 g/100mL. The strain was identified as Saccharomyces cerevisiae by morphological analysis and 26S rDNA sequencing.

Keywords: high temperature resistance; high-producing alcohol yeast; Saccharomyces cerevisiae

doi: 10.3969/j.issn.1674-506X.2023.01-011

收稿日期：2022-09-11

基金项目：贵州省科技支撑计划项目（黔科合支撑［2018］2134）；贵州省科技计划项目（黔科合支撑［2019］2342）

作者简介：曹云（1995-），女，硕士研究生。研究方向：微生物与发酵。

*通信作者：曹文涛（1967-），男，教授。研究方向：白酒资源化利用及酿酒微生物应用研究。

引用格式:曹云,曹文涛,王晓丹,等.耐高温高产酒精酵母菌的筛选鉴定及特性研究[J].食品与发酵科技,2023,59(1):83-89.